In addition, long-lasting epigenetic changes are evident in the offspring of women who experience famine during the first trimester of pregnancy, but are absent in those who either do not experience famine or who experience it at a later gestational period.
Gene Chip Mouse Genome 430 2.0 Arrays (Affymetrix, Santa Clara, CA, USA) were used to profile the transcriptome according to the manufacturer’s instructions.
The protocols for fatty acid analysis are described elsewhere and in the Supplementary Methods.
Behavior was assessed using the following tests: open-field test, tail-suspension test, Y-maze test, prepulse inhibition (PPI) test, forced swim test, light and dark box test, elevated plus-maze test, home cage activity test and MK-801 sensitivity test at 2 to 6 month old.
Total phospholipid fractions were separated by thin-layer chromatography.
The content of each fatty acid was expressed as the percentage area of total fatty acids.Considering the essential role of PUFA, we hypothesized that the compromised availability of dietary PUFA may affect early neurodevelopment and thereby predispose to the development of schizophrenia.